Design, Synthesis, and Biological Evaluation of 1,2,4-Triazole Derivatives as Potential Antimicrobial Agents

In this study, we aimed to develop new biologically active compounds with antibacterial properties. 4-amino-5-methyl-2H-1,2,4-triazol-3(4H)-one (1) was converted the corresponding Schiff bases (2) reaction a 4-anis aldehyde. Acetic acid ethyl esters containing [1,2,4] triazole ring (3) were synthesized by condensation of bromoacetate in basic media. The hydrazine hydrate led formation hydrazides (4). hydrazide (4) phenyl isothio - and isocyanate produced carbothioamide (5a) carboxamide (5b). treatment and. (5b) 1,2,4-triazole (6a, 6b) compounds, respectively. reactions ( (6b) norfloxacin ciprofloxacin presence formaldehyde afforded Mannich bases( 7a, 7b). structural assignments based on elemental analysis spectral (IR, 1H-NMR, 13C-NMR) data. All newly screened for their antimicrobial activity. vitro activities evaluated against pathogenic microorganisms, 7a 7b found most effective